Overview

  • Product name

  • Description

    Goat polyclonal to HIC1
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human HIC1 aa 415-429 (internal sequence). (NP_001091672.1).
    Sequence:

    HLEGYPCPHLAYGEP


    Database link: Q14526

  • Positive control

    • WB: A549 and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 77 kDa).

Target

  • Function

    Transcriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
  • Tissue specificity

    Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
    Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hic 1 antibody
    • HIC ZBTB transcriptional repressor 1 antibody
    • Hic-1 antibody
    • Hic1 antibody
    • HIC1_HUMAN antibody
    • Hypermethylated in cancer 1 antibody
    • Hypermethylated in cancer 1 protein antibody
    • ZBTB29 antibody
    • Zinc finger and BTB domain-containing protein 29 antibody
    • ZNF901 antibody
    see all

Images

  • All lanes : Anti-HIC1 antibody (ab223486) at 1 µg/ml

    Lane 1 : A549 (human lung carcinoma cell line) cell lysate (in RIPA buffer)
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 77 kDa



    Primary incubation was 1 hour.

References

ab223486 has not yet been referenced specifically in any publications.

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