Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223486 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 77 kDa).|
FunctionTranscriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
Tissue specificityUbiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
Contains 1 BTB (POZ) domain.
Contains 5 C2H2-type zinc fingers.
modificationsAcetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
- Information by UniProt
- Hic 1 antibody
- HIC ZBTB transcriptional repressor 1 antibody
- Hic-1 antibody
All lanes : Anti-HIC1 antibody (ab223486) at 1 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) cell lysate (in RIPA buffer)
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 77 kDa
Primary incubation was 1 hour.
ab223486 has not yet been referenced specifically in any publications.