Key features and details
- Rabbit polyclonal to HIC1
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HIC1 antibody
See all HIC1 primary antibodies
DescriptionRabbit polyclonal to HIC1
SpecificityThis antibody is specific for HIC1.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
- Jurkat cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab49326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500.
FunctionTranscriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
Tissue specificityUbiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
Contains 1 BTB (POZ) domain.
Contains 5 C2H2-type zinc fingers.
modificationsAcetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
- Information by UniProt
- Hic 1 antibody
- HIC ZBTB transcriptional repressor 1 antibody
- Hic-1 antibody
Lane 1 : MARKER
Lane 2 : Anti-HIC1 antibody (ab49326) at 0.25 µg/ml
Lane 2 : Jurkat cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
ab49326 has been referenced in 1 publication.
- Zhang X et al. Ferroptosis is governed by differential regulation of transcription in liver cancer. Redox Biol 24:101211 (2019). PubMed: 31108460