Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12901] to HID1/DMC1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-HID1/DMC1 antibody [EPR12901]
See all HID1/DMC1 primary antibodies
DescriptionRabbit monoclonal [EPR12901] to HID1/DMC1
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment within Human HID1/DMC1. The exact sequence is proprietary.
Database link: Q8IV36
- A549, MCF-7, fetal brain, human stomach, SK-BR-3 and HT-29 lysates; MCF-7 cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as CQ028
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 89 kDa.|
|ICC/IF||1/100 - 1/250.|
FunctionMay play an important role in the development of cancers in a broad range of tissues.
Tissue specificityExpressed in heart, skeletal muscle, colon, spleen, kidney, liver, small intestine and lung. Highest expression is seen in brain and placenta. Loss of expression is seen in some breast, cervical, hepatocellular, lung, thyroid, gastric and renal cell-cancer lines.
Sequence similaritiesBelongs to the UPF0663 family.
- Information by UniProt
- C17orf28 antibody
- Chromosome 17 open reading frame 28 antibody
- CQ028_HUMAN antibody
Immunofluorescent staining of MCF7 cells using labeling HID1/DMC1 with ab175926 at 1/100 dilution (red). DAPI nuclear staining (blue).
All lanes : Anti-HID1/DMC1 antibody [EPR12901] (ab175926) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Fetal brain cell lysate
Lane 4 : Human stomach lysate
Lane 5 : SK-BR-3 cell lysate
Lane 6 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDa
ab175926 has not yet been referenced specifically in any publications.