Recombinant Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free (ab249893)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12901] to HID1/DMC1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free
See all HID1/DMC1 primary antibodies -
Description
Rabbit monoclonal [EPR12901] to HID1/DMC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human HID1/DMC1. The exact sequence is proprietary.
Database link: Q8IV36 -
General notes
ab249893 is the carrier-free version of ab175926.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12901 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
Target
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Function
May play an important role in the development of cancers in a broad range of tissues. -
Tissue specificity
Expressed in heart, skeletal muscle, colon, spleen, kidney, liver, small intestine and lung. Highest expression is seen in brain and placenta. Loss of expression is seen in some breast, cervical, hepatocellular, lung, thyroid, gastric and renal cell-cancer lines. -
Sequence similarities
Belongs to the UPF0663 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 283987 Human
- Omim: 605752 Human
- SwissProt: Q8IV36 Human
- Unigene: 11067 Human
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Alternative names
- C17orf28 antibody
- Chromosome 17 open reading frame 28 antibody
- CQ028_HUMAN antibody
see all
Images
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All lanes : Anti-HID1/DMC1 antibody [EPR12901] (ab175926) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Fetal brain cell lysate
Lane 4 : Human stomach lysate
Lane 5 : SK-BR-3 cell lysate
Lane 6 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDaThis data was developed using ab175926, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249893 has not yet been referenced specifically in any publications.