Key features and details
- Rabbit polyclonal to HIF-1-alpha
- Suitable for: IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HIF-1-alpha antibody
See all HIF-1 alpha primary antibodies
DescriptionRabbit polyclonal to HIF-1-alpha
Tested applicationsSuitable for: IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Carp, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human HIF-1-alpha aa 775-826 (C terminal).
- Mouse squamous cell carcinoma tissue; HeLa cells.
Concentration is optimized for IHC and not determined.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesab114977 was affinity purified using an epitope specific to HIF-1-alpha immobilized on solid support.
Our Abpromise guarantee covers the use of ab114977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.|
|ICC||1/100 - 1/500.|
FunctionFunctions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Tissue specificityExpressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
DomainContains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
modificationsIn normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
- Information by UniProt
- ARNT interacting protein antibody
- ARNT-interacting protein antibody
- Basic helix loop helix PAS protein MOP1 antibody
ab114977 at 1:250 dilution staining HIF-1-alpha in Formaldehyde-fixed asynchronous hypoxic HeLa cells by Immunocytochemistry.
Detection: DAB staining.
ab114977 at 1:250 dilution staining HIF-1-alpha in Formalin-fixed, Paraffin-embedded Mouse squamous cell carcinoma tissue by Immunohistochemistry.
Detection: DAB staining.
ab114977 has been referenced in 9 publications.
- Martano M et al. Evaluation of Hypoxia-Inducible Factor-1 Alpha (HIF-1a) in Equine Sarcoid: An Immunohistochemical and Biochemical Study. Pathogens 9:N/A (2020). PubMed: 31947661
- Toullec A et al. HIF-1a Deletion in the Endothelium, but Not in the Epithelium, Protects From Radiation-Induced Enteritis. Cell Mol Gastroenterol Hepatol 5:15-30 (2018). IHC ; Human . PubMed: 29276749
- Peng JK et al. ?ypoxia-inducible factor 1-a promotes colon cell proliferation and migration by upregulating AMPK-related protein kinase 5 under hypoxic conditions. Oncol Lett 15:3639-3645 (2018). PubMed: 29467884
- Chen Z et al. MiR-3662 suppresses hepatocellular carcinoma growth through inhibition of HIF-1a-mediated Warburg effect. Cell Death Dis 9:549 (2018). PubMed: 29748591
- Sikes KJ et al. TGF-b1 or hypoxia enhance glucose metabolism and lactate production via HIF1A signaling in tendon cells. Connect Tissue Res 59:458-471 (2018). PubMed: 29447016
- Iwamoto H et al. Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance. Cell Metab 28:104-117.e5 (2018). PubMed: 29861385
- Liu L et al. The impact of high co-expression of Sp1 and HIF1a on prognosis of patients with hepatocellular cancer. Oncol Lett 12:504-512 (2016). IHC-P ; Human . PubMed: 27347172
- Chen F et al. Proteome Differences in Placenta and Endometrium between Normal and Intrauterine Growth Restricted Pig Fetuses. PLoS One 10:e0142396 (2015). WB ; Pig . PubMed: 26554841
- Ronkainen VP et al. Hypoxia-inducible factor 1-induced G protein-coupled receptor 35 expression is an early marker of progressive cardiac remodelling. Cardiovasc Res N/A:N/A (2013). PubMed: 24095869