Overview

  • Product name

  • Description

    Rabbit polyclonal to HIF-1 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HIF-1 alpha aa 351-400 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    IFSLQQTECV LKPVESSDMK MTQLFTKVES EDTSSLFDKL KKEPDALTLL


    Database link: Q16665

  • Positive control

    • IHC-P: Human cervical carcinoma tissue. WB: Mouse heart lysates; rat brain and spleen tissue lysates; HL60 cell lysate. ICC/IF: KLN 205 cells.
  • General notes

    HIF-1 alpha can be a difficult target to work with so we have compiled a summary of all the important information you need to know including useful tips. This can be found in the protocols tab or alternatively click here to download the English version and here to download the Mandarin.

Properties

Applications

Our Abpromise guarantee covers the use of ab216842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/100 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
ICC/IF 1/50 - 1/200.
Flow Cyt 1/20 - 1/100.

Target

  • Function

    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
  • Tissue specificity

    Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Domain

    Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
  • Post-translational
    modifications

    In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
    S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
    Requires phosphorylation for DNA-binding.
    Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
    Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARNT interacting protein antibody
    • ARNT-interacting protein antibody
    • Basic helix loop helix PAS protein MOP1 antibody
    • Basic-helix-loop-helix-PAS protein MOP1 antibody
    • bHLHe78 antibody
    • Class E basic helix-loop-helix protein 78 antibody
    • HIF 1A antibody
    • HIF 1alpha antibody
    • HIF-1-alpha antibody
    • HIF-1alpha antibody
    • HIF-alpha antibody
    • HIF1 A antibody
    • HIF1 Alpha antibody
    • HIF1 antibody
    • HIF1-alpha antibody
    • HIF1A antibody
    • HIF1A_HUMAN antibody
    • hifla antibody
    • Hypoxia inducible factor 1 alpha antibody
    • Hypoxia inducible factor 1 alpha isoform I.3 antibody
    • Hypoxia inducible factor 1 alpha subunit antibody
    • Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
    • Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody
    • Hypoxia inducible factor1alpha antibody
    • Hypoxia-inducible factor 1-alpha antibody
    • Member of PAS protein 1 antibody
    • Member of PAS superfamily 1 antibody
    • Member of the PAS Superfamily 1 antibody
    • MOP 1 antibody
    • MOP1 antibody
    • PAS domain-containing protein 8 antibody
    • PASD 8 antibody
    • PASD8 antibody
    see all

Images

  • All lanes : Anti-HIF-1 alpha antibody (ab216842) at 1/200 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse large intestine lysate

    Secondary
    All lanes : Goat anti-rabbit IgG antibody (H+L), HRP at 1/3000 dilution

    Predicted band size: 92 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling HIF-1-alpha with ab216842 at 1/300 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunofluorescent analysis of KLN 205 cells labeling HIF-1-alpha with ab216842 at 1/50 dilution followed by an FITC conjugated secondary antibody.

  • All lanes : Anti-HIF-1 alpha antibody (ab216842) at 1/1000 dilution

    Lanes 1-2 : Rat spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Predicted band size: 92 kDa

  • Anti-HIF-1 alpha antibody (ab216842) at 1/300 dilution + HL60 cell lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution

    Predicted band size: 92 kDa

  • Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling HIF-1 aplha with ab216842 at a 1/100 dilution for 30 minutes (green) compared to control cells (blue) and an isotype control (orange).

References

This product has been referenced in:

  • Wiafe B  et al. Mesenchymal stem cell therapy inhibited inflammatory and profibrotic pathways induced by partial bladder outlet obstruction and prevented high-pressure urine storage. J Pediatr Urol N/A:N/A (2019). Read more (PubMed: 30967358) »
  • Zhang X  et al. Effect of microRNA-21 on hypoxia-inducible factor-1a in orthodontic tooth movement and human periodontal ligament cells under hypoxia. Exp Ther Med 17:2830-2836 (2019). Read more (PubMed: 30930976) »
See all 6 Publications for this product

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