Western blot abreview for Anti-HIF-1 alpha antibody - ChIP Grade

Inconclusive
Abreviews
Application
Western blot
Sample
Notothenia coriiceps Cell lysate - other (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
CTmax
Specification
heart
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1:1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Sigma anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: ??? kDa
Positive control
human HIF-1a nuclear extract

Additional data

Abcam response
Thank you for participating in our AbTrial testing program. Based on these results, it appears the antibody may not be cross-reacting with this species. We appreciate the feedback.

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Verified customer

Submitted Jan 13 2017

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