Western blot abreview for Anti-HIF-1 alpha antibody - ChIP Grade

Inconclusive
Abreviews
Application
Western blot
Sample
Chaenocephalus aceratus Cell lysate - other (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
CTmax
Specification
heart
Blocking step
BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blotto
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: sigma anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Positive control
human HIF-1a nuclear extract

Additional data

Additional Notes
No bands with C. aceratus
Abcam response
Thank you for participating in our AbTrial testing program. Based on these results, it appears the antibody may not be cross-reacting with this species. We appreciate the feedback.

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Verified customer

Submitted Jan 13 2017

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