Anti-HIF-2-alpha antibody [ep190b] (ab8365)
Key features and details
- Mouse monoclonal [ep190b] to HIF-2-alpha
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-HIF-2-alpha antibody [ep190b]
See all HIF-2-alpha primary antibodies -
Description
Mouse monoclonal [ep190b] to HIF-2-alpha -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Human -
Immunogen
Fusion protein (proprietary-tag) corresponding to Human HIF-2-alpha aa 500-650.
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Positive control
- WB: Hypoxic A549 and HeLa cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ep190b -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. Abcam recommends using a 3% milk block with this product. |
Target
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Function
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. -
Tissue specificity
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells. -
Involvement in disease
Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsIn normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2034 Human
- Entrez Gene: 29452 Rat
- Omim: 603349 Human
- SwissProt: Q99814 Human
- SwissProt: Q9JHS1 Rat
- Unigene: 468410 Human
- Unigene: 55138 Rat
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Alternative names
- Basic helix loop helix PAS protein MOP2 antibody
- Basic-helix-loop-helix-PAS protein MOP2 antibody
- bHLHe73 antibody
see all
Images
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All lanes : Anti-HIF-2-alpha antibody [ep190b] (ab8365) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa treated with 0.5mM DFO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab8365 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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HIF-2-alpha detected in hypoxic Human lysate using ab8365. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.
Protocols
References (64)
ab8365 has been referenced in 64 publications.
- Morin A et al. TET-Mediated Hypermethylation Primes SDH-Deficient Cells for HIF2a-Driven Mesenchymal Transition. Cell Rep 30:4551-4566.e7 (2020). PubMed: 32234487
- Guo X et al. Whole-exome sequencing and immunohistochemistry findings in von Hippel-Lindau disease. Mol Genet Genomic Med 7:e880 (2019). PubMed: 31317677
- Cho C et al. Cirsium japonicum var. maackii and apigenin block Hif-2a-induced osteoarthritic cartilage destruction. J Cell Mol Med 23:5369-5379 (2019). PubMed: 31148341
- Polívka J et al. IDH1 mutation is associated with lower expression of VEGF but not microvessel formation in glioblastoma multiforme. Oncotarget 9:16462-16476 (2018). IHC-P ; Human . PubMed: 29662659
- Zhang T et al. Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis. BMC Cancer 18:536 (2018). WB ; Human . PubMed: 29739381