Overview

  • Product name

    Anti-HIF Prolyl Hydroxylases antibody
    See all HIF Prolyl Hydroxylases primary antibodies
  • Description

    Rabbit polyclonal to HIF Prolyl Hydroxylases
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 317-512 of Human HIF Prolyl Hydroxylases (UniProt ID: Q9NXG6).

  • Positive control

    • A549 and HeLa whole cell lysates; MCF7 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function

    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • Tissue specificity

    Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
  • Sequence similarities

    Contains 2 EF-hand domains.
    Contains 1 Fe2OG dioxygenase domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EGLN4 antibody
    • FLJ20262 antibody
    • HIF prolyl hydroxylase PH4 antibody
    • HIF-PH4 antibody
    • HIF-prolyl hydroxylase 4 antibody
    • HIFPH4 antibody
    • HPH-4 antibody
    • Hypoxia inducible factor prolyl 4 hydroxylase antibody
    • Hypoxia inducible factor prolyl hydroxylase antibody
    • Hypoxia-inducible factor prolyl hydroxylase 4 antibody
    • P4H TM antibody
    • P4H with transmembrane domain antibody
    • P4H-TM antibody
    • P4htm antibody
    • P4HTM_HUMAN antibody
    • PH 4 antibody
    • PH4 antibody
    • PHD4 antibody
    • Proline 4 hydroxylase antibody
    • Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) antibody
    • Prolyl hydroxlase domain containing 4 antibody
    • Transmembrane prolyl 4-hydroxylase antibody
    see all

Images

  • Anti-HIF Prolyl Hydroxylases antibody (ab154375) at 1/2000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labeling HIF Prolyl Hydroxylases using ab154375 at 1/250 dilution.

References

ab154375 has not yet been referenced specifically in any publications.

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