Key features and details
- Rabbit polyclonal to HIF Prolyl Hydroxylases
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HIF Prolyl Hydroxylases antibody
See all HIF Prolyl Hydroxylases primary antibodies
DescriptionRabbit polyclonal to HIF Prolyl Hydroxylases
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 317-512 of Human HIF Prolyl Hydroxylases (UniProt ID: Q9NXG6).
- A549 and HeLa whole cell lysates; MCF7 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Tissue specificityWidely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
Sequence similaritiesContains 2 EF-hand domains.
Contains 1 Fe2OG dioxygenase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- EGLN4 antibody
- FLJ20262 antibody
- HIF prolyl hydroxylase PH4 antibody
ab154375 has not yet been referenced specifically in any publications.