Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734)


  • Product name
    Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)]
    See all HIF Prolyl Hydroxylases primary antibodies
  • Description
    Rabbit monoclonal [EPR3664(2)] to HIF Prolyl Hydroxylases
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HIF Prolyl Hydroxylases aa 50-150. The exact sequence is proprietary.

  • Positive control
    • 293T, U87-MG, HeLa, HepG2, and SH-SY5Y cell lysates, Human brain tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.


Associated products


Our Abpromise guarantee covers the use of ab109734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
    • Tissue specificity
      Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
    • Sequence similarities
      Contains 2 EF-hand domains.
      Contains 1 Fe2OG dioxygenase domain.
    • Post-translational
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EGLN4 antibody
      • FLJ20262 antibody
      • HIF prolyl hydroxylase PH4 antibody
      • HIF-PH4 antibody
      • HIF-prolyl hydroxylase 4 antibody
      • HIFPH4 antibody
      • HPH-4 antibody
      • Hypoxia inducible factor prolyl 4 hydroxylase antibody
      • Hypoxia inducible factor prolyl hydroxylase antibody
      • Hypoxia-inducible factor prolyl hydroxylase 4 antibody
      • P4H TM antibody
      • P4H with transmembrane domain antibody
      • P4H-TM antibody
      • P4htm antibody
      • P4HTM_HUMAN antibody
      • PH 4 antibody
      • PH4 antibody
      • PHD4 antibody
      • Proline 4 hydroxylase antibody
      • Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) antibody
      • Prolyl hydroxlase domain containing 4 antibody
      • Transmembrane prolyl 4-hydroxylase antibody
      see all


    • All lanes : Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109734 at a dilution of 1/100.


    ab109734 has not yet been referenced specifically in any publications.

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