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    hif1-beta-antibody-2b10-ab2771.pdf

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Cardiovascular Hypoxia HIF
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Anti-HIF1 beta antibody [2B10] (ab2771)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (18)

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Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)

Key features and details

  • Mouse monoclonal [2B10] to HIF1 beta
  • Suitable for: Flow Cyt, WB, ICC/IF, IHC-P, IP
  • Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish, African green monkey
  • Isotype: IgG1

You may also be interested in

Primary
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HRP Anti-6X His tag® antibody (ab1187)
Protein
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Recombinant Human HIF1 beta protein (ab157907)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-HIF1 beta antibody [2B10]
    See all HIF1 beta primary antibodies
  • Description

    Mouse monoclonal [2B10] to HIF1 beta
  • Host species

    Mouse
  • Specificity

    Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish, African green monkey
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to Human HIF1 beta aa 771-789.
    Sequence:

    NSYNNEEFPDLTMFPPFSE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    2B10
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Hypoxia
    • HIF
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Epigenetics and Nuclear Signaling
    • Cardiovascular/Immune
    • Hypoxia
    • HIF
    • Cancer
    • Invasion/microenvironment
    • Hypoxia
    • HIF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human HIF1 beta protein (ab157907)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (6)
Use at an assay dependent concentration.
EMSA
Use at an assay dependent concentration.
Gel Shift Assay
Use at an assay dependent concentration.
ICC/IF
1/1000.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP (1)
Use at an assay dependent concentration.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration.
EMSA
Use at an assay dependent concentration.
Gel Shift Assay
Use at an assay dependent concentration.
ICC/IF
1/1000.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

Target

  • Function

    Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P27540 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281010 Cow
    • Entrez Gene: 405 Human
    • Entrez Gene: 11863 Mouse
    • Entrez Gene: 100008996 Rabbit
    • Entrez Gene: 25242 Rat
    • Entrez Gene: 64277 Zebrafish
    • Omim: 126110 Human
    • SwissProt: Q9BE97 Cow
    • SwissProt: P27540 Human
    • SwissProt: P53762 Mouse
    • SwissProt: O02748 Rabbit
    • SwissProt: P41739 Rat
    • Unigene: 632446 Human
    • Unigene: 250265 Mouse
    • Unigene: 10520 Rat
    see all
  • Alternative names

    • arnT antibody
    • ARNT protein antibody
    • ARNT_HUMAN antibody
    • Aryl hydrocarbon receptor nuclear translocator antibody
    • bHLHe2 antibody
    • Class E basic helix-loop-helix protein 2 antibody
    • Dioxin receptor antibody
    • Dioxin receptor nuclear translocator antibody
    • Drnt antibody
    • HIF 1 beta antibody
    • HIF 1beta antibody
    • HIF-1-beta antibody
    • HIF1-beta antibody
    • HIF1B antibody
    • HIF1beta antibody
    • Hypoxia Inducible Factor 1 antibody
    • Hypoxia inducible factor 1 beta antibody
    • Hypoxia-inducible factor 1-beta antibody
    • nuclear translocator antibody
    • Tango antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)

    Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in A2058 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)

    Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in HeLa cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)

    Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in U251 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)
    IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)
    Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)
    Overlay histogram showing PC12 cells stained with ab2771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab2771? Please let us know so that we can cite the reference in this datasheet.

ab2771 has been referenced in 18 publications.

  • Wu CS  et al. ASC modulates HIF-1a stability and induces cell mobility in OSCC. Cell Death Dis 11:721 (2020). PubMed: 32883954
  • Zhao Y  et al. Aryl hydrocarbon receptor nuclear translocator promotes the proliferation and invasion of clear cell renal cell carcinoma cells potentially by affecting the glycolytic pathway. Oncol Lett 20:56 (2020). PubMed: 32793310
  • Li C  et al. microRNA-222-Mediated VHL Downregulation Facilitates Retinoblastoma Chemoresistance by Increasing HIF1a Expression. Invest Ophthalmol Vis Sci 61:9 (2020). PubMed: 32756923
  • Fu YC & Xin ZM Inhibited corneal neovascularization in rabbits following corneal alkali burn by double-target interference for VEGF and HIF-1a. Biosci Rep 39:N/A (2019). PubMed: 30355648
  • Chen WC  et al. Aryl hydrocarbon receptor modulates stroke-induced astrogliosis and neurogenesis in the adult mouse brain. J Neuroinflammation 16:187 (2019). PubMed: 31606043
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunoprecipitation abreview for Anti-HIF1 beta antibody [2B10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
500 µg
Specification
293T
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 30 2007

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