- Datasheet
- Specific References (11)
- Protocols
Overview
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Product nameAnti-HIF1 beta antibody [2B10]
See all HIF1 beta primary antibodies -
DescriptionMouse monoclonal [2B10] to HIF1 beta
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Host speciesMouse
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SpecificityDetects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, ICC, IHC-P, IP, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish, African green monkey
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide corresponding to Human HIF1 beta aa 771-789.
Sequence:NSYNNEEFPDLTMFPPFSE
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityAffinity purified
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ClonalityMonoclonal
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Clone number2B10
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| EMSA | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| WB | 1/4000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa). |
Target
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FunctionRequired for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
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Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 281010 Cow
- Entrez Gene: 405 Human
- Entrez Gene: 11863 Mouse
- Entrez Gene: 100008996 Rabbit
- Entrez Gene: 25242 Rat
- Entrez Gene: 64277 Zebrafish
- Omim: 126110 Human
- SwissProt: Q9BE97 Cow
see all -
Alternative names
- arnT antibody
- ARNT protein antibody
- ARNT_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-218998-ab2771iccif3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in A2058 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-218997-ab2771iccif2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in HeLa cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-218996-ab2771iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in U251 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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![Western blot - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771_1.jpg)
Western blot - Anti-HIF1 beta antibody [2B10] (ab2771)This image is courtesy of an anonymous AbreviewAnti-HIF1 beta antibody [2B10] (ab2771) at 1/2000 dilution + Human U2OS cells - whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 90 kDa (why is the actual band size different from the predicted?)
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 5 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/HIF1-beta-Primary-antibodies-ab2771-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/HIF1-beta-Primary-antibodies-ab2771-2.jpg)
Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)Overlay histogram showing PC12 cells stained with ab2771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References
This product has been referenced in:
- Shih JW et al. Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1a co-activator driving oral cancer progression. Nat Commun 8:15874 (2017). WB ; Human . Read more (PubMed: 28639619) »
- Guo F et al. Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell Res 27:967-988 (2017). ICC/IF ; Mouse . Read more (PubMed: 28621329) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"