Key features and details
- Rabbit polyclonal to HIF1 beta
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-HIF1 beta antibody
See all HIF1 beta primary antibodies
DescriptionRabbit polyclonal to HIF1 beta
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Cow
Recombinant fragment (His-T7-tag) corresponding to Human HIF1 beta aa 624-789. (Expressed in E.coli).
QISRHSNPTQGATPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTP SSFSSMSLPGAPTASPGAAAYPSLTNRGSNFAPETGQTAGQFQTRTAEGV GVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQSNSY NNEEFPDLTMFPPFSE
Database link: P27540
- WB: Recombinant human HIF1 beta protein; Human serum; HepG2 cell lysate; Pig kidney lysate; Rat placenta lysate. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232964 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 87 kDa.|
FunctionRequired for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
- Information by UniProt
- arnT antibody
- ARNT protein antibody
- ARNT_HUMAN antibody
All lanes : Anti-HIF1 beta antibody (ab232964) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Pig kidney lysate
Lane 4 : Rat placenta lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 87 kDa
Anti-HIF1 beta antibody (ab232964) at 2 µg/ml + Recombinant human HIF1 beta protein
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 87 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells labelling HIF1 beta (green) using ab232964 at 20 µg/ml in ICC/IF, followed by FITC staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232964 has not yet been referenced specifically in any publications.