Overview

  • Product name

  • Description

    Rabbit polyclonal to HIF1 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human HIF1 beta aa 1-50. The exact sequence is proprietary. (NP_001659.1).
    Database link: P27540

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
  • General notes

    ab245420 has not performed satisfactorily when used for WB of HIF1 beta in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HIF1 beta.

Properties

Applications

Our Abpromise guarantee covers the use of ab245420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • arnT antibody
      • ARNT protein antibody
      • ARNT_HUMAN antibody
      • Aryl hydrocarbon receptor nuclear translocator antibody
      • bHLHe2 antibody
      • Class E basic helix-loop-helix protein 2 antibody
      • Dioxin receptor antibody
      • Dioxin receptor nuclear translocator antibody
      • Drnt antibody
      • HIF 1 beta antibody
      • HIF 1beta antibody
      • HIF-1-beta antibody
      • HIF1-beta antibody
      • HIF1B antibody
      • HIF1beta antibody
      • Hypoxia Inducible Factor 1 antibody
      • Hypoxia inducible factor 1 beta antibody
      • Hypoxia-inducible factor 1-beta antibody
      • nuclear translocator antibody
      • Tango antibody
      see all

    Images

    • HIF1 beta was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245420 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245420 at 0.4 µg/ml.

      Lane 1: ab245420 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for HIF1 beta using ab245420 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    References

    ab245420 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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