Product nameAnti-HIF1AN / FIH-1 antibody
See all HIF1AN / FIH-1 primary antibodies
DescriptionRabbit polyclonal to HIF1AN / FIH-1
SpecificityThe expected molecular weight of FIH is 40 kDa. A band corresponding to this size is seen on the western blot analysis, and is competed away by the addition of FIH blocking peptide. A smaller band is seen, which is also competed away by the blocking peptide. In the A431 extract a nonspecific band of approximately 50 kDa is seen, which is not diminished by the addition of blocking peptide.
Tested applicationsSuitable for: IHC-FoFr, ICC/IF, WBmore details
Species reactivityReacts with: Chicken, Human
Synthetic peptide corresponding to Human HIF1AN/ FIH-1 aa 250 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human HIF1AN / FIH-1 protein (ab86916) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293 and Hela Nuclear lysate.
Previously labelled as HIF1AN.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab12289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Can be blocked with FIH peptide (ab12306).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
Sequence similaritiesContains 1 JmjC domain.
- Information by UniProt
- DKFZp762F1811 antibody
- Factor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibody
All lanes : Anti-HIF1AN / FIH-1 antibody (ab12289) at 1/500 dilution
Lane 1 : Hela nuclear extract
Lanes 2 & 7 : Hela whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : Hek293 cell lysate
Lane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/ml
Lane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/ml
Lane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/ml
Lane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 5 minutes
Lane 1: 20
g of HeLa nuclear extract. µ
Lane 2: 20
g of HeLa whole cell lysate. µ
Lane 3: 20
g of A431 whole cell lysate. µ
Lane 4: 20
ICC/IF image of ab12289 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab12289, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
This product has been referenced in:
- Sallais J et al. Factor inhibiting HIF1-A novel target of SUMOylation in the human placenta. Oncotarget 8:114002-114018 (2017). Read more (PubMed: 29371964) »
- Yang Y et al. A novel fumarate hydratase-deficient HLRCC kidney cancer cell line, UOK268: a model of the Warburg effect in cancer. Cancer Genet 205:377-90 (2012). Read more (PubMed: 22867999) »