• Product name

    Anti-HIF1AN/FIH-1 antibody
    See all HIF1AN/FIH-1 primary antibodies
  • Description

    Rabbit polyclonal to HIF1AN/FIH-1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human HIF1AN/FIH-1.
    Database link: Q9NWT6

  • Positive control

    • MCF7, K562, U937, 293T cell lysates; mouse heart;and skeletal muscle lysates.
  • General notes

    Previously labelled as HIF1AN.



Our Abpromise guarantee covers the use of ab187524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/200.


  • Function

    Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
  • Sequence similarities

    Contains 1 JmjC domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp762F1811 antibody
    • Factor inhibiting HIF-1 antibody
    • Factor inhibiting HIF1 antibody
    • FIH 1 antibody
    • FIH-1 antibody
    • FIH1 antibody
    • FLJ20615 antibody
    • FLJ22027 antibody
    • HIF1AN antibody
    • HIF1N_HUMAN antibody
    • Hypoxia inducible factor 1 alpha inhibitor antibody
    • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
    • Hypoxia inducible factor asparagine hydroxylase antibody
    • Hypoxia-inducible factor 1-alpha inhibitor antibody
    • Hypoxia-inducible factor asparagine hydroxylase antibody
    • Peptide aspartate beta dioxygenase antibody
    see all


  • All lanes : Anti-HIF1AN/FIH-1 antibody (ab187524) at 1/500 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : U937 lysate
    Lane 4 : 293T cell lysate
    Lane 5 : Mouse heart lysate
    Lane 6 : Mouse skeletal muscle lysate

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling HIF1AN/FIH-1 with ab187524 at 1/200. Magnification: 400x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling HIF1AN/FIH-1 with ab187524 at 1/200. Magnification: 400x.

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187524. Blue DAPI for nuclear staining.


This product has been referenced in:

  • Liu Y  et al. Adipose-derived exosomes deliver miR-23a/b to regulate tumor growth in hepatocellular cancer by targeting the VHL/HIF axis. J Physiol Biochem 75:391-401 (2019). Read more (PubMed: 31321740) »
  • Johansson E  et al. CD44 Interacts with HIF-2a to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells. Cell Rep 20:1641-1653 (2017). WB ; Mouse . Read more (PubMed: 28813675) »
See all 3 Publications for this product

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