Overview

  • Product name

    Anti-HIF1AN/FIH-1 antibody
    See all HIF1AN/FIH-1 primary antibodies
  • Description

    Rabbit polyclonal to HIF1AN/FIH-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human HIF1AN/FIH-1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NWT6

  • Positive control

    • ICC/IF: HeLa and MCF7 cells. WB: HCT 116 and MCF7 whole cell lysate. IHC-P: U373 xenograft tissue.
  • General notes

    Previously labelled as HIF1AN.

Properties

Applications

Our Abpromise guarantee covers the use of ab227550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
  • Sequence similarities

    Contains 1 JmjC domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp762F1811 antibody
    • Factor inhibiting HIF-1 antibody
    • Factor inhibiting HIF1 antibody
    • FIH 1 antibody
    • FIH-1 antibody
    • FIH1 antibody
    • FLJ20615 antibody
    • FLJ22027 antibody
    • HIF1AN antibody
    • HIF1N_HUMAN antibody
    • Hypoxia inducible factor 1 alpha inhibitor antibody
    • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
    • Hypoxia inducible factor asparagine hydroxylase antibody
    • Hypoxia-inducible factor 1-alpha inhibitor antibody
    • Hypoxia-inducible factor asparagine hydroxylase antibody
    • Peptide aspartate beta dioxygenase antibody
    see all

Images

  • All lanes : Anti-HIF1AN/FIH-1 antibody (ab227550) at 1/1000 dilution

    Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    10% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF1AN/FIH-1 (green) using ab227550 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: phalloidin, a cytoskeleton marker, diluted at 1/200 dilution.

    Blue: Hoechst 33342 staining.

  • MCF7 (human breast adenocarcinoma cell line) cells stained for HIF1AN/FIH-1 (green) using ab227550 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded U373 xenograft tissue stained for HIF1AN/FIH-1 using ab227550 at 1/500 dilution in immunohistochemical analysis.

References

ab227550 has not yet been referenced specifically in any publications.

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