Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Human
Product nameAnti-HIF1AN/FIH-1 antibody [EPR3658] - BSA and Azide free
See all HIF1AN/FIH-1 primary antibodies
DescriptionRabbit monoclonal [EPR3658] to HIF1AN/FIH-1 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human HIF1AN/FIH-1 aa 1-100. The exact sequence is proprietary.
- Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.
ab214440 is the carrier-free version of ab92498 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab214440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
Sequence similaritiesContains 1 JmjC domain.
- Information by UniProt
- DKFZp762F1811 antibody
- Factor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibody
This data was developed using ab92498, the same antibody clone in a different buffer formulation.
ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab214440 has not yet been referenced specifically in any publications.