Anti-HIF3 alpha / IPAS antibody - Neuronal Marker (ab223524)

Overview

  • Product name
    Anti-HIF3 alpha / IPAS antibody - Neuronal Marker
    See all HIF3 alpha / IPAS primary antibodies
  • Description
    Goat polyclonal to HIF3 alpha / IPAS - Neuronal Marker
  • Host species
    Goat
  • Specificity
    ab223524 is expected to recognize isoform a and c (NP_690007.1; NP_690008.2).
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HIF3 alpha/ IPAS aa 9-19 (N terminal) (Cysteine residue). (NP_690008.2).
    Sequence:

    QDHRSTTELRKEKS-C


    Database link: Q9Y2N7

  • Positive control
    • IHC-P: Human kidney tissue.
  • General notes

    Previously labelled as HIF3 alpha. 

Properties

Applications

Our Abpromise guarantee covers the use of ab223524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.
  • Tissue specificity
    Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications
    In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.
  • Cellular localization
    Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic-helix-loop-helix-PAS protein MOP7 antibody
    • bHLHe17 antibody
    • Class E basic helix-loop-helix protein 17 antibody
    • HIF 3A antibody
    • HIF 3A4 antibody
    • HIF-3-alpha antibody
    • HIF3 alpha antibody
    • HIF3-alpha antibody
    • HIF3-alpha-1 antibody
    • HIF3A antibody
    • HIF3A_HUMAN antibody
    • Hypoxia Inducible Factor 3 alpha antibody
    • Hypoxia inducible factor 3 alpha subunit antibody
    • Hypoxia inducible factor three alpha antibody
    • Hypoxia-inducible factor 3-alpha antibody
    • Inhibitory PAS domain protein antibody
    • IPAS antibody
    • Member of PAS protein 7 antibody
    • MOP7 antibody
    • PAS domain-containing protein 7 antibody
    • PASD7 antibody
    see all

Images

  • Paraffin-embedded human kidney tissue stained for HIF3 alpha / IPAS using ab223524 at 4 µg/ml in immunohistochemical analysis, followed by HRP-staining.

References

ab223524 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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