Anti-HIGD1A antibody (ab117456)
Key features and details
- Rabbit polyclonal to HIGD1A
- Suitable for: ICC/IF, ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIGD1A antibody -
Description
Rabbit polyclonal to HIGD1A -
Host species
Rabbit -
Specificity
ab117456 is predicted to not cross-react with HIG2. -
Tested applications
Suitable for: ICC/IF, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to 19 amino acids near the N terminal of Human HIGD1A (NP_001093138).
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Positive control
- 293 cell lysate.
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General notes
Avoid repeated freeze thaw cycles and exposure to prolonged high temperatures.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab117456 was purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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ICC |
Use a concentration of 2.5 µg/ml.
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WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
ICC
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa. |
Target
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Sequence similarities
Contains 1 HIG1 domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25994 Human
- SwissProt: Q9Y241 Human
- Unigene: 593134 Human
- Unigene: 728813 Human
- Unigene: 7917 Human
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Alternative names
- Hig1 antibody
- HIG1 domain family member 1A antibody
- HIG1 domain family, member 1A antibody
see all
Images
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Lane 1 : Anti-HIGD1A antibody (ab117456) at 0.5 µg/ml
Lane 2 : Anti-HIGD1A antibody (ab117456) at 1 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 12 kDa -
Immunofluorescence of HIGD1A in 293 cells tissue with ab117456 at 10 ug/mL.
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Immunocytochemical analysis of 293 cells, staining HIGD1A with ab117456 at 2.5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab117456 has not yet been referenced specifically in any publications.