The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab168061 binds to Human CD40. ab168061 induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).
< 0.100 Eu/µg
>90% by SDS-PAGE. ab168061 is purified by multi-step chromatography.
ab168061 is a high-activity construct in which two trimeric CD40L molecules are artificially linked via the collagen domain of Adiponectin. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with 100µl sterile water for a final concentration of 0.1mg/ml. After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles. Further dilutions should be made with medium containing 5% fetal calf serum.
CD40 antigen ligand
CD40 ligand, soluble form
T B cell activating molecule
T-cell antigen Gp39
TNF-related activation protein
Tumor necrosis factor (ligand) superfamily member 5
Tumor necrosis factor ligand superfamily member 5
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Specifically expressed on activated CD4+ T-lymphocytes.
Involvement in disease
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Belongs to the tumor necrosis factor family.
The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated.
SDS-PAGE - High activity fusion construct of human soluble TRAP/CD40L (ab168061)
SDS-PAGE analysis of ab168061.
Functional Studies - High activity fusion construct of human soluble TRAP/CD40L (ab168061)
B cell lymphocyte activation by ab168061.
Method: Peripheral Blood Mononuclear Cells (PBMCs) were incubated at 37°C, 5% CO2 for 48 hours in 48 well plates (1 x 106 cells/well). Each well contained 200µl serum free test media with serially diluted ab168061. After treatment, cells were washed and dual-stained with mouse anti-human CD19–PE and mouse anti-human CD86–APC and analyzed by flow cytometry. The data are presented as the percent of CD19 positive B cells that co-stain as CD86 positive, at each concentration of ab168061.
has not yet been referenced specifically in any publications.
Publishing research using ab168061? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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