Anti-HINT1 antibody (ab226363)
Key features and details
- Rabbit polyclonal to HINT1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HINT1 antibody
See all HINT1 primary antibodies -
Description
Rabbit polyclonal to HINT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Orangutan -
Immunogen
Synthetic peptide within Human HINT1 aa 76-126. The exact sequence is proprietary.
Database link: P49773 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226363 was affinity purified using an epitope specific to HINT1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/2000 - 1/10000. Predicted molecular weight: 14 kDa. |
Target
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Function
Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the HINT family.
Contains 1 HIT domain. -
Domain
The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide. -
Cellular localization
Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization. - Information by UniProt
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Database links
- Entrez Gene: 327693 Cow
- Entrez Gene: 3094 Human
- Entrez Gene: 15254 Mouse
- Entrez Gene: 100009302 Rabbit
- Omim: 601314 Human
- SwissProt: P62958 Cow
- SwissProt: P49773 Human
- SwissProt: P70349 Mouse
see all -
Alternative names
- Adenosine 5' monophosphoramidase antibody
- Adenosine 5''-monophosphoramidase antibody
- HINT 1 antibody
see all
Images
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All lanes : Anti-HINT1 antibody (ab226363) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa
Exposure time: 75 secondsCell lysates were prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab226363 has not yet been referenced specifically in any publications.