Anti-HINT1 antibody (ab226363)

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Western blot - Anti-HINT1 antibody (ab226363)

    Key features and details

    • Rabbit polyclonal to HINT1
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-HINT1 antibody
      See all HINT1 primary antibodies

    • Description

      Rabbit polyclonal to HINT1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rabbit, Cow, Orangutan

    • Immunogen

      Synthetic peptide within Human HINT1 aa 76-126. The exact sequence is proprietary.
      Database link: P49773

    • Positive control

      • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab226363 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/2000 - 1/10000. Predicted molecular weight: 14 kDa.

    Target

    • Function

      Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the HINT family.
      Contains 1 HIT domain.

    • Domain

      The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.

    • Cellular localization

      Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adenosine 5' monophosphoramidase antibody
      • Adenosine 5''-monophosphoramidase antibody
      • HINT 1 antibody
      • HINT1 antibody
      • HINT1_HUMAN antibody
      • Histidine triad nucleotide binding protein 1 antibody
      • Histidine triad nucleotide-binding protein 1 antibody
      • PKCI 1 antibody
      • PKCI-1 antibody
      • PKCI1 antibody
      • PRKCNH1 antibody
      • Protein kinase C inhibitor 1 antibody
      • Protein kinase C interacting protein 1 antibody
      • Protein kinase C-interacting protein 1 antibody
      see all

    Images

    • Western blot - Anti-HINT1 antibody (ab226363)
      Western blot - Anti-HINT1 antibody (ab226363)
      All lanes : Anti-HINT1 antibody (ab226363) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 14 kDa


      Exposure time: 75 seconds


      Cell lysates were prepared using NETN lysis buffer.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226363? Please let us know so that we can cite the reference in this datasheet.

    ab226363 has not yet been referenced specifically in any publications.

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