Product nameAnti-HINT1 antibody
See all HINT1 primary antibodies
DescriptionRabbit polyclonal to HINT1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Orangutan
Synthetic peptide within Human HINT1 aa 76-126. The exact sequence is proprietary.
Database link: P49773
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226363 was affinity purified using an epitope specific to HINT1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 14 kDa.|
FunctionHydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the HINT family.
Contains 1 HIT domain.
DomainThe histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
Cellular localizationCytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
- Information by UniProt
- Adenosine 5' monophosphoramidase antibody
- Adenosine 5''-monophosphoramidase antibody
- HINT 1 antibody
All lanes : Anti-HINT1 antibody (ab226363) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa
Exposure time: 75 seconds
Cell lysates were prepared using NETN lysis buffer.
ab226363 has not yet been referenced specifically in any publications.