Anti-HINT1 antibody (ab226363)

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Overview

  • Product name

    Anti-HINT1 antibody
    See all HINT1 primary antibodies

  • Description

    Rabbit polyclonal to HINT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cow, Orangutan

  • Immunogen

    Synthetic peptide within Human HINT1 aa 76-126. The exact sequence is proprietary.
    Database link: P49773

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab226363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 14 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the HINT family.
      Contains 1 HIT domain.

    • Domain

      The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.

    • Cellular localization

      Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adenosine 5' monophosphoramidase antibody
      • Adenosine 5''-monophosphoramidase antibody
      • HINT 1 antibody
      • HINT1 antibody
      • HINT1_HUMAN antibody
      • Histidine triad nucleotide binding protein 1 antibody
      • Histidine triad nucleotide-binding protein 1 antibody
      • PKCI 1 antibody
      • PKCI-1 antibody
      • PKCI1 antibody
      • PRKCNH1 antibody
      • Protein kinase C inhibitor 1 antibody
      • Protein kinase C interacting protein 1 antibody
      • Protein kinase C-interacting protein 1 antibody
      see all

    Images

    • Western blot - Anti-HINT1 antibody (ab226363)
      Western blot - Anti-HINT1 antibody (ab226363)
      All lanes : Anti-HINT1 antibody (ab226363) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 14 kDa


      Exposure time: 75 seconds


      Cell lysates were prepared using NETN lysis buffer.

    References

    ab226363 has not yet been referenced specifically in any publications.

    Publishing research using ab226363? Please let us know so that we can cite the reference in this datasheet.

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