Overview

  • Product name

    Anti-HINT1 antibody [EPR5108]
    See all HINT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5108] to HINT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HINT1. The exact sequence is proprietary.

  • Positive control

    • 293T, MCF7, HepG2 and Jurkat cell lysates; Human breast carcinoma and Human colonic adenocarcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the HINT family.
    Contains 1 HIT domain.
  • Domain

    The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • Cellular localization

    Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenosine 5' monophosphoramidase antibody
    • Adenosine 5''-monophosphoramidase antibody
    • HINT 1 antibody
    • HINT1 antibody
    • HINT1_HUMAN antibody
    • Histidine triad nucleotide binding protein 1 antibody
    • Histidine triad nucleotide-binding protein 1 antibody
    • PKCI 1 antibody
    • PKCI-1 antibody
    • PKCI1 antibody
    • PRKCNH1 antibody
    • Protein kinase C inhibitor 1 antibody
    • Protein kinase C interacting protein 1 antibody
    • Protein kinase C-interacting protein 1 antibody
    see all

Images

  • All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 14 kDa

  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.
  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.

References

This product has been referenced in:

  • Zhou Y  et al. Altered Light Conditions Contribute to Abnormalities in Emotion and Cognition Through HINT1 Dysfunction in C57BL/6 Mice. Front Behav Neurosci 12:110 (2018). Read more (PubMed: 29937721) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab124912.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up