Recombinant
RabMAb

Recombinant Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

Overview

  • Product name

    Anti-HINT1 antibody [EPR5108] - BSA and Azide free
    See all HINT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5108] to HINT1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HINT1. The exact sequence is proprietary.

  • General notes

    Ab240007 is the carrier-free version of ab124912. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240007 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

Target

  • Function

    Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the HINT family.
    Contains 1 HIT domain.
  • Domain

    The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • Cellular localization

    Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenosine 5' monophosphoramidase antibody
    • Adenosine 5''-monophosphoramidase antibody
    • HINT 1 antibody
    • HINT1 antibody
    • HINT1_HUMAN antibody
    • Histidine triad nucleotide binding protein 1 antibody
    • Histidine triad nucleotide-binding protein 1 antibody
    • PKCI 1 antibody
    • PKCI-1 antibody
    • PKCI1 antibody
    • PRKCNH1 antibody
    • Protein kinase C inhibitor 1 antibody
    • Protein kinase C interacting protein 1 antibody
    • Protein kinase C-interacting protein 1 antibody
    see all

Images

  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124912).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124912).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab240007 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240007.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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