Key features and details
- Rabbit polyclonal to HINT3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HINT3 antibody
DescriptionRabbit polyclonal to HINT3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: RT4 cell lysate. ICC: A431 cells. IHC-P: Human prostate, cerebral cortex and colon tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionHydrolyzes phosphoramidate and acyl-adenylate substrates.
Sequence similaritiesBelongs to the HINT family.
Contains 1 HIT domain.
Cellular localizationCytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus.
- Information by UniProt
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
Immunohistochemical analysis of human colon tissue labeling HINT3 with ab121960 at a 1/200 dilution.
Immunocytochemistry of A431 (Human epidermoid carcinoma) cells labeling HINT3 with ab121960 at 0.25 ug/ml.
Anti-HINT3 antibody (ab121960) at 0.04 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysate
Western blot analysis of RT-4 cells labeling HINT3 with ab121960 at 0.04 ug/ml.
Immunohistochemical analysis of human prostate tissue labeling HINT3 with ab121960 at a 1/200 dilution.
Immunohistochemical analysis of human cerebral cortex tissue labeling HINT3 with ab121960 at a 1/200 dilution.
ab121960 has not yet been referenced specifically in any publications.