Anti-HINT3 antibody (ab121960)
Key features and details
- Rabbit polyclonal to HINT3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HINT3 antibody -
Description
Rabbit polyclonal to HINT3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).
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Positive control
- WB: RT4 cell lysate. ICC: A431 cells. IHC-P: Human prostate, cerebral cortex and colon tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Hydrolyzes phosphoramidate and acyl-adenylate substrates. -
Sequence similarities
Belongs to the HINT family.
Contains 1 HIT domain. -
Cellular localization
Cytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 135114 Human
- Omim: 609998 Human
- SwissProt: Q9NQE9 Human
- Unigene: 72325 Human
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Alternative names
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)
Immunohistochemical analysis of human colon tissue labeling HINT3 with ab121960 at a 1/200 dilution.
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Immunocytochemistry - Anti-HINT3 antibody (ab121960)Immunocytochemistry of A431 (Human epidermoid carcinoma) cells labeling HINT3 with ab121960 at 0.25 ug/ml.
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Western blot - Anti-HINT3 antibody (ab121960)Anti-HINT3 antibody (ab121960) at 0.04 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysateWestern blot analysis of RT-4 cells labeling HINT3 with ab121960 at 0.04 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)Immunohistochemical analysis of human prostate tissue labeling HINT3 with ab121960 at a 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)Immunohistochemical analysis of human cerebral cortex tissue labeling HINT3 with ab121960 at a 1/200 dilution.
Protocols
Datasheets and documents
References (0)
ab121960 has not yet been referenced specifically in any publications.
