Anti-HINT3 antibody (ab121960)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HINT3 antibody -
Description
Rabbit polyclonal to HINT3 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).
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Positive control
- WB: RT4, U-251 MG, Human liver and Human tonsil lysates IHC-P: Human colon tissue ICC/IF: U-2 OS cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Hydrolyzes phosphoramidate and acyl-adenylate substrates. -
Sequence similarities
Belongs to the HINT family.
Contains 1 HIT domain. -
Cellular localization
Cytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 135114 Human
- Omim: 609998 Human
- SwissProt: Q9NQE9 Human
- Unigene: 72325 Human
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Alternative names
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
Images
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Western blot - Anti-HINT3 antibody (ab121960)All lanes : Anti-HINT3 antibody (ab121960) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
10-20% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)ab121960, at a 1/100 dilution, staining HINT3 in paraffin emebedded Human colon tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-HINT3 antibody (ab121960)ab121960, at 4 µg/ml, staining HINT3 in Human cell line U-2 OS by Immunofluorescence.
Protocols
Datasheets and documents
References
ab121960 has not yet been referenced specifically in any publications.
