Product nameAnti-HINT3 antibody
DescriptionRabbit polyclonal to HINT3
Tested applicationsSuitable for: IP, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: RT4, U-251 MG, Human liver and Human tonsil lysates IHC-P: Human colon tissue ICC/IF: U-2 OS cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100|
FunctionHydrolyzes phosphoramidate and acyl-adenylate substrates.
Sequence similaritiesBelongs to the HINT family.
Contains 1 HIT domain.
Cellular localizationCytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus.
- Information by UniProt
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
All lanes : Anti-HINT3 antibody (ab121960) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
10-20% SDS PAGE
ab121960, at a 1/100 dilution, staining HINT3 in paraffin emebedded Human colon tissue by Immunohistochemistry.
ab121960, at 4 µg/ml, staining HINT3 in Human cell line U-2 OS by Immunofluorescence.
ab121960 has not yet been referenced specifically in any publications.