Anti-HINT3 antibody (ab121960)
Key features and details
- Rabbit polyclonal to HINT3
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HINT3 antibody -
Description
Rabbit polyclonal to HINT3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).
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Positive control
- WB: RT4 cell lysate. ICC: A431 cells. IHC-P: Human prostate, cerebral cortex and colon tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.25 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC
Use a concentration of 0.25 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Hydrolyzes phosphoramidate and acyl-adenylate substrates. -
Sequence similarities
Belongs to the HINT family.
Contains 1 HIT domain. -
Cellular localization
Cytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 135114 Human
- Omim: 609998 Human
- SwissProt: Q9NQE9 Human
- Unigene: 72325 Human
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Alternative names
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
Images
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Immunohistochemical analysis of human colon tissue labeling HINT3 with ab121960 at a 1/200 dilution.
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Immunocytochemistry of A431 (Human epidermoid carcinoma) cells labeling HINT3 with ab121960 at 0.25 ug/ml.
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Anti-HINT3 antibody (ab121960) at 0.04 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysate
Western blot analysis of RT-4 cells labeling HINT3 with ab121960 at 0.04 ug/ml.
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Immunohistochemical analysis of human prostate tissue labeling HINT3 with ab121960 at a 1/200 dilution.
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Immunohistochemical analysis of human cerebral cortex tissue labeling HINT3 with ab121960 at a 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121960 has not yet been referenced specifically in any publications.