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Rabbit polyclonal to HINT3
antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).
- WB: RT4, U-251 MG, Human liver and Human tonsil lysates
IHC-P: Human colon tissue
ICC/IF: U-2 OS cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
Hydrolyzes phosphoramidate and acyl-adenylate substrates.
Belongs to the HINT family.
Contains 1 HIT domain.
Cytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus.
Information by UniProt
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
Western blot - Anti-HINT3 antibody (ab121960)
All lanes : Anti-HINT3 antibody (ab121960) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
10-20% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)
ab121960, at a 1/100 dilution, staining HINT3 in paraffin emebedded Human colon tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-HINT3 antibody (ab121960)
ab121960, at 4 µg/ml, staining HINT3 in Human cell line U-2 OS by Immunofluorescence.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"