Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10814] to HIP1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-HIP1 antibody [EPR10814]
See all HIP1 primary antibodies
DescriptionRabbit monoclonal [EPR10814] to HIP1
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
- HCT-116, HeLa, Jurkat and A549 cell lysates; HeLa cells.
Previously labelled as Huntingtin Interacting Protein HIP1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab181238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).Can be blocked with HIP1 peptide (ab188140).|
|IP||1/20 - 1/40.|
FunctionPlays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors.
Tissue specificityUbiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer.
Involvement in diseaseNote=A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed.
Sequence similaritiesBelongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain.
DomainThe pseudo DED region (pDED) mediates the interaction with IFT57.
Binds F-actin via the talin-like I/LWEQ domain.
Cellular localizationCytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR.
- Information by UniProt
- 2610109B09Rik antibody
- A930014B11Rik antibody
- E130315I21Rik antibody
All lanes : Anti-HIP1 antibody [EPR10814] (ab181238) at 1/2000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling HIP1 with ab181238 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181238 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
ab181238 has been referenced in 1 publication.
- Yang Q et al. Endocytic Adaptor Protein HIP1R Controls Intracellular Trafficking of Epidermal Growth Factor Receptor in Neuronal Dendritic Development. Front Mol Neurosci 11:447 (2018). PubMed: 30574069