Overview

  • Product name

    Anti-HIP1 antibody [EPR10814]
    See all HIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10814] to HIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human HIP1 aa 950 to the C-terminus. The exact sequence is proprietary.
    Database link: O00291
    (Peptide available as ab188140)

  • Positive control

    • HCT-116, HeLa, Jurkat and A549 cell lysates; HeLa cells.
  • General notes

     

     

    Previously labelled as Huntingtin Interacting Protein HIP1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10814
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
IP 1/20 - 1/40.

Target

  • Function

    Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors.
  • Tissue specificity

    Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer.
  • Involvement in disease

    Note=A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed.
  • Sequence similarities

    Belongs to the SLA2 family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
    Contains 1 I/LWEQ domain.
  • Domain

    The pseudo DED region (pDED) mediates the interaction with IFT57.
    Binds F-actin via the talin-like I/LWEQ domain.
  • Cellular localization

    Cytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610109B09Rik antibody
    • A930014B11Rik antibody
    • E130315I21Rik antibody
    • HIP 1 antibody
    • HIP I antibody
    • HIP-1 antibody
    • HIP-I antibody
    • hip1 antibody
    • HIP1/PDGFRB fusion gene antibody
    • HIP1/PDGFRB fusion gene, included antibody
    • HIP1_HUMAN antibody
    • HIPI antibody
    • Huntingtin interacting protein 1 antibody
    • Huntingtin Interacting Protein HIP1 antibody
    • Huntingtin-interacting protein 1 antibody
    • Huntingtin-interacting protein I antibody
    • ILWEQ antibody
    • KIAA4113 antibody
    • MGC126506 antibody
    • MGC27616 antibody
    • mKIAA4113 antibody
    see all

Images

  • All lanes : Anti-HIP1 antibody [EPR10814] (ab181238) at 1/2000 dilution

    Lane 1 : HCT-116 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 116 kDa

  • Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling HIP1 with ab181238 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot analysis of immunoprecipitation pellet from A549 cell lysate  immunoprecipitated using ab181238 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

References

This product has been referenced in:

  • Yang Q  et al. Endocytic Adaptor Protein HIP1R Controls Intracellular Trafficking of Epidermal Growth Factor Receptor in Neuronal Dendritic Development. Front Mol Neurosci 11:447 (2018). Read more (PubMed: 30574069) »
See 1 Publication for this product

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