Key features and details
- Rabbit polyclonal to HIP14
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HIP14 antibody
See all HIP14 primary antibodies
DescriptionRabbit polyclonal to HIP14
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human cerebral cortex, caudate putamen, testis and placenta tissue. ICC/IF: U-2 OS cells. WB: HIP14 overexpression HEK-293T lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab253017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceZDHHC17 is a neuronal palmitoyl transferase. Palmitoylation is critical for trafficking and function of signaling molecules, neurotransmitter receptors, and synaptic scaffolding proteins in neurons. ZDHHC17 causes cellular transformation. It has been shown that mRNA encoding ZDHHC17 is up-regulated in a number of types of human tumors, thus ZDHHC17 and other PATs (palmitoyl acyltransferases) are potential targets for new anticancer drugs.
Cellular localizationGolgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
- DHHC 17 antibody
- DHHC domain containing 17 antibody
- HIP14 antibody
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for HIP14 with ab253017 at a 1/200 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2OS (Human brain glioma cell line) cells stained for HIP14 (green) using ab253017 at 4 µg/ml in ICC/IF.
All lanes : Anti-HIP14 antibody (ab253017) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : HIP14 overexpression HEK-293T lysate
Formalin-fixed, paraffin-embedded human caudate putamen tissue stained for HIP14 with ab253017 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for HIP14 with ab253017 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human placenta tissue stained for HIP14 with ab253017 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for HIP14 when incubated with ab253017 at a 1/200 dilution (negative control).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253017 has not yet been referenced specifically in any publications.