Product nameAnti-HIP1R antibody
See all HIP1R primary antibodies
DescriptionRabbit polyclonal to HIP1R
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Giant Panda
Synthetic peptide within Human HIP1R aa 300-350. The exact sequence is proprietary.
Database link: O75146
- WB: HeLa, HEK-293T and Jurkat whole cell lysate (ab7899). IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226197 was affinity purified using an epitope specific to HIP1R immobilized on solid support.
Our Abpromise guarantee covers the use of ab226197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 70,119 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
Tissue specificityBrain, heart, kidney, pancreas, and liver, but not in lung or placenta.
Sequence similaritiesBelongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain.
DomainBinds F-actin via the talin-like I/LWEQ domain.
Cellular localizationCytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region.
- Information by UniProt
- FLJ14000 antibody
- FLJ27022 antibody
- HIP-12 antibody
All lanes : Anti-HIP1R antibody (ab226197) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 70,119 kDa
Exposure time: 10 seconds
Cell lysates were prepared using NETN lysis buffer.
HIP1R was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20% of IP loaded) with ab226197 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226197 at 0.4 µg/ml.
Lane 1: ab226197 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226197 has not yet been referenced specifically in any publications.