Anti-HIP1R antibody (ab226197)
Key features and details
- Rabbit polyclonal to HIP1R
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIP1R antibody
See all HIP1R primary antibodies -
Description
Rabbit polyclonal to HIP1R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Giant Panda
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Immunogen
Synthetic peptide within Human HIP1R aa 300-350. The exact sequence is proprietary.
Database link: O75146 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate (ab7899). IP: HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226197 was affinity purified using an epitope specific to HIP1R immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 70,119 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. -
Tissue specificity
Brain, heart, kidney, pancreas, and liver, but not in lung or placenta. -
Sequence similarities
Belongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain. -
Domain
Binds F-actin via the talin-like I/LWEQ domain. -
Cellular localization
Cytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 100613620 Chimpanzee
- Entrez Gene: 100060573 Horse
- Entrez Gene: 9026 Human
- Entrez Gene: 29816 Mouse
- Entrez Gene: 707396 Rhesus monkey
- Omim: 605613 Human
- SwissProt: O75146 Human
- SwissProt: Q9JKY5 Mouse
see all -
Alternative names
- FLJ14000 antibody
- FLJ27022 antibody
- HIP-12 antibody
see all
Images
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Western blot - Anti-HIP1R antibody (ab226197)All lanes : Anti-HIP1R antibody (ab226197) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 70,119 kDa
Exposure time: 10 secondsCell lysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-HIP1R antibody (ab226197)HIP1R was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20% of IP loaded) with ab226197 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226197 at 0.4 µg/ml.
Lane 1: ab226197 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab226197 has been referenced in 1 publication.
- Saralamma VVG et al. Comparative proteomic analysis uncovers potential biomarkers involved in the anticancer effect of Scutellarein in human gastric cancer cells. Oncol Rep 44:939-958 (2020). PubMed: 32705238
