Key features and details
- Rabbit polyclonal to HIP1R
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-HIP1R antibody
See all HIP1R primary antibodies
DescriptionRabbit polyclonal to HIP1R
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human HIP1R aa 771-1012. Expressed in E. coli. N-terminal tags.
SLDVRQEELGAVVDKEMAATSAAIEDAVRRIEDMMNQARHASSGVKLEVN ERILNSCTDLMKAIRLLVTTSTSLQKEIVESGRGAATQQEFYAKNSRWTE GLISASKAVGWGATQLVEAADKVVLHTGKYEELIVCSHEIAASTAQLVAA SKVKANKHSPHLSRLQECSRTVNERAANVVASTKSGQEQIEDRDTMDFSG LSLIKLKKQEMETQVRVLELEKTLEAERMRLGELRKQHYVLA
Database link: O75146
- WB: Recombinant human HIP1R protein; pig kidney and brain tissue lysate; HeLa whole cell lysate. ICC/IF: HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 119 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionComponent of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
Tissue specificityBrain, heart, kidney, pancreas, and liver, but not in lung or placenta.
Sequence similaritiesBelongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain.
DomainBinds F-actin via the talin-like I/LWEQ domain.
Cellular localizationCytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region.
- Information by UniProt
- FLJ14000 antibody
- FLJ27022 antibody
- HIP-12 antibody
All lanes : Anti-HIP1R antibody (ab232808) at 3 µg/ml
Lane 1 : Pig kidney tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Pig brain tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 119 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIP1R (green) using ab232808 at 20 μg/ml in ICC/IF. FITC stained.
Anti-HIP1R antibody (ab232808) at 2 µg/ml + Recombinant human HIP1R protein
Predicted band size: 119 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232808 has not yet been referenced specifically in any publications.