Key features and details
- Rabbit polyclonal to HIP1R
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-HIP1R antibody
See all HIP1R primary antibodies
DescriptionRabbit polyclonal to HIP1R
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human HIP1R aa 304-487.
VIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPNGSVKDDRD LQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQ KALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEK HSELVHVHAELLRKNADTAKQLTVTQQSQEEVAR
Database link: O75146
- WB: Rat brain tissue lysate. IHC-P: Human pancreatic and kidney tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000.|
FunctionComponent of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
Tissue specificityBrain, heart, kidney, pancreas, and liver, but not in lung or placenta.
Sequence similaritiesBelongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain.
DomainBinds F-actin via the talin-like I/LWEQ domain.
Cellular localizationCytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region.
- Information by UniProt
- FLJ14000 antibody
- FLJ27022 antibody
- HIP-12 antibody
Anti-HIP1R antibody (ab234991) at 1/500 dilution + Rat brain tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 120 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kideny tissue stained for HIP1R using ab234991 at 1/800 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The staining was perfromed on a Leica BondTM system. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Paraffin-embedded human pancreas tissue stained for HIP1R using ab234991 at 1/800 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The staining was performed on a Leica BondTM system. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234991 has not yet been referenced specifically in any publications.