Recombinant Anti-HIP1R antibody [EPR9437] (ab140608)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9437] to HIP1R
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-HIP1R antibody [EPR9437]
See all HIP1R primary antibodies -
Description
Rabbit monoclonal [EPR9437] to HIP1R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HIP1R aa 1000-1100 (C terminal). The exact sequence is proprietary.
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Positive control
- Human fetal kidney and HeLa cell lysates; Human kidney tissue; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9437 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 119 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/250. |
Target
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Function
Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. -
Tissue specificity
Brain, heart, kidney, pancreas, and liver, but not in lung or placenta. -
Sequence similarities
Belongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain. -
Domain
Binds F-actin via the talin-like I/LWEQ domain. -
Cellular localization
Cytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 9026 Human
- Omim: 605613 Human
- SwissProt: O75146 Human
- Unigene: 524815 Human
- Unigene: 714965 Human
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Alternative names
- FLJ14000 antibody
- FLJ27022 antibody
- HIP-12 antibody
see all
Images
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All lanes : Anti-HIP1R antibody [EPR9437] (ab140608) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 119 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HIP1R with ab140608 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labelling HIP1R with ab140608 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using ab140608 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab140608 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140608 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140608 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab140608 has not yet been referenced specifically in any publications.