Recombinant
RabMAb

Recombinant Anti-HIP1R antibody [EPR9437] (ab140608)

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Overview

  • Product name

    Anti-HIP1R antibody [EPR9437]
    See all HIP1R primary antibodies

  • Description

    Rabbit monoclonal [EPR9437] to HIP1R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HIP1R aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney and HeLa cell lysates; Human kidney tissue; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 119 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.

    • Tissue specificity

      Brain, heart, kidney, pancreas, and liver, but not in lung or placenta.

    • Sequence similarities

      Belongs to the SLA2 family.
      Contains 1 ENTH (epsin N-terminal homology) domain.
      Contains 1 I/LWEQ domain.

    • Domain

      Binds F-actin via the talin-like I/LWEQ domain.

    • Cellular localization

      Cytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region.
    • Information by UniProt

    • Database links

      • Alternative names

        • FLJ14000 antibody
        • FLJ27022 antibody
        • HIP-12 antibody
        • Hip1 related antibody
        • HIP1-related protein antibody
        • HIP12 antibody
        • HIP1R antibody
        • HIP1R_HUMAN antibody
        • HIP3 antibody
        • Huntingtin Interacting Protein 1 Related antibody
        • Huntingtin interacting protein 12 antibody
        • Huntingtin-interacting protein 1-related protein antibody
        • Huntingtin-interacting protein 12 antibody
        • ILWEQ antibody
        • KIAA0655 antibody
        • MGC47513 antibody
        see all

      Images

      • Western blot - Anti-HIP1R antibody [EPR9437] (ab140608)
        Western blot - Anti-HIP1R antibody [EPR9437] (ab140608)
        All lanes : Anti-HIP1R antibody [EPR9437] (ab140608) at 1/1000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 119 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)

        Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HIP1R with ab140608 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunofluorescent analysis of HeLa cells labelling HIP1R with ab140608 at 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)

        Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using ab140608 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)

        Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab140608 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)

        Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140608 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (ab140608)

        Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140608 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      ab140608 has not yet been referenced specifically in any publications.

      Publishing research using ab140608? Please let us know so that we can cite the reference in this datasheet.

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