Product nameHIP1R overexpression 293T lysate (whole cell)
ab94268 is a 293T cell transfected lysate in which Human HIP1R has been transiently over-expressed using a pCMV-HIP1R plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. Tissue specificity: Brain, heart, kidney, pancreas, and liver, but not in lung or placenta. Similarity: Belongs to the SLA2 family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 1 I/LWEQ domain. Domain: Binds F-actin via the talin-like I/LWEQ domain.
Our Abpromise guarantee covers the use of ab94268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94268 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-HIP1R antibody (ab88468) at 1/500 dilution
Lane 1 :
HIP1R overexpression 293T lysate (whole cell) (ab94268)
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94268 has not yet been referenced specifically in any publications.