Overview

  • Product name
    Anti-HIP2 antibody - C-terminal
    See all HIP2 primary antibodies
  • Description
    Rabbit polyclonal to HIP2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human HIP2 aa 150-200 (C terminal). The exact sequence is proprietary.
    Sequence:

    HVYAGAPVSSP EYTKKIENLC AMGFDRNAVI VALSSKSWDV ETATELLLSN


    Database link: P61086

  • Positive control
    • 293T, HeLa, Jurkat, TCMK1 and NIH 3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab177521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.

Target

  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • Tissue specificity
    Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • Post-translational
    modifications
    Sumoylation at Lys-14 impairs catalytic activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 25K antibody
    • E2(25K) antibody
    • HIP-2 antibody
    • Huntingtin interacting protein 2 antibody
    • Huntingtin-interacting protein 2 antibody
    • HYPG antibody
    • LIG antibody
    • UBC1 antibody
    • UBE2K antibody
    • UBE2K_HUMAN antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 25 kDa antibody
    • ubiquitin conjugating enzyme E2K antibody
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin-conjugating enzyme E2 K antibody
    • Ubiquitin-conjugating enzyme E2(25K) antibody
    • Ubiquitin-conjugating enzyme E2-25 kDa antibody
    • Ubiquitin-conjugating enzyme E2-25K antibody
    • Ubiquitin-protein ligase antibody
    see all

Images

  • Lane 1: 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated with ab177521 at 6 μg/mg lysate.
    Secondary: Anti-rabbit HRP conjugated antibody

    Lane 2: 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated with control IgG at 6 μg/mg lysate.
    Secondary: Anti-rabbit HRP conjugated antibody

  • All lanes : Anti-HIP2 antibody - C-terminal (ab177521) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Anti-rabbit HRP conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 22 kDa


    Exposure time: 10 seconds

References

ab177521 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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