Overview

  • Product name

  • Description

    Rabbit polyclonal to HIP2/LIG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human HIP2/LIG (internal sequence). The exact sequence is proprietary.
    Database link: P61086

  • Positive control

    • WB: Mouse brain tisssue lysate; HCT 116 whole cell lysate. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as HIP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • Tissue specificity

    Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • Post-translational
    modifications

    Sumoylation at Lys-14 impairs catalytic activity.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2 25K antibody
    • E2(25K) antibody
    • HIP-2 antibody
    • Huntingtin interacting protein 2 antibody
    • Huntingtin-interacting protein 2 antibody
    • HYPG antibody
    • LIG antibody
    • UBC1 antibody
    • UBE2K antibody
    • UBE2K_HUMAN antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 25 kDa antibody
    • ubiquitin conjugating enzyme E2K antibody
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin-conjugating enzyme E2 K antibody
    • Ubiquitin-conjugating enzyme E2(25K) antibody
    • Ubiquitin-conjugating enzyme E2-25 kDa antibody
    • Ubiquitin-conjugating enzyme E2-25K antibody
    • Ubiquitin-protein ligase antibody
    see all

Images

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIP2/LIG (green) using ab227268 at 1/200 dilution in ICC/IF.

    Blue: Hoechst 33342 staining (lower panel).

  • Anti-HIP2/LIG antibody (ab227268) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 22 kDa



    12% SDS-PAGE

  • Anti-HIP2/LIG antibody (ab227268) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 22 kDa



    12% SDS-PAGE

References

ab227268 has not yet been referenced specifically in any publications.

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