Overview

  • Product name

  • Description

    Goat polyclonal to HIP2/LIG
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Chicken, Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human HIP2/LIG aa 187-200 (C terminal).
    Sequence:

    C-SWDVETATELLLSN

  • Positive control

    • Jurkat and Human Liver lysates.
  • General notes

     This product was previously labelled as HIP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab37917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • Tissue specificity

    Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • Post-translational
    modifications

    Sumoylation at Lys-14 impairs catalytic activity.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2 25K antibody
    • E2(25K) antibody
    • HIP-2 antibody
    • Huntingtin interacting protein 2 antibody
    • Huntingtin-interacting protein 2 antibody
    • HYPG antibody
    • LIG antibody
    • UBC1 antibody
    • UBE2K antibody
    • UBE2K_HUMAN antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 25 kDa antibody
    • ubiquitin conjugating enzyme E2K antibody
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin-conjugating enzyme E2 K antibody
    • Ubiquitin-conjugating enzyme E2(25K) antibody
    • Ubiquitin-conjugating enzyme E2-25 kDa antibody
    • Ubiquitin-conjugating enzyme E2-25K antibody
    • Ubiquitin-protein ligase antibody
    see all

Images

  • Anti-HIP2/LIG antibody (ab37917) at 0.5 µg/ml + Jurkat lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size: 23 kDa



    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Anti-HIP2/LIG antibody (ab37917) at 1/1000 dilution + Cell lysate prepared from chicken DT40 lymphoblast cell line at 30 µg

    Secondary
    Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Samples blocked with 5% milk for 30 minutes at room temperature.

    Gel 12.5-15% run under denaturing conditions.

    See Abreview

  • ab37917 at 3.7 µg/ml staining HIP2/LIG in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab37917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Chicken Cell lysate - other (Soluble Fraction)
Gel Running Conditions
Reduced Denaturing (12.5-15%)
Loading amount
30 µg
Specification
Soluble Fraction
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 25 2010

Application
Western blot
Sample
Human Cell lysate - other (Soluble Fraction)
Loading amount
30 µg
Specification
Soluble Fraction
Gel Running Conditions
Reduced Denaturing (12.5-15%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 20 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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