Product nameAnti-HIP2/LIG antibody [EP1144Y]
See all HIP2/LIG primary antibodies
DescriptionRabbit monoclonal [EP1144Y] to HIP2/LIG
Tested applicationsSuitable for: WB, IP, ICCmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human HIP2/LIG (N terminal). The exact sequence is proprietary.
- Daudi, Jurkat, HepG2 and human brain cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as HIP2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).|
|ICC||1/100 - 1/250.|
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
Tissue specificityExpressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
Contains 1 UBA domain.
modificationsSumoylation at Lys-14 impairs catalytic activity.
- Information by UniProt
- E2 25K antibody
- E2(25K) antibody
- HIP-2 antibody
All lanes : Anti-HIP2/LIG antibody [EP1144Y] (ab68162) at 1/500 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : human brain cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
ab68162 has not yet been referenced specifically in any publications.