• Product name

    Anti-HIP2/LIG antibody [EP1144Y]
    See all HIP2/LIG primary antibodies
  • Description

    Rabbit monoclonal [EP1144Y] to HIP2/LIG
  • Host species

  • Tested applications

    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HIP2/LIG (N terminal). The exact sequence is proprietary.

  • Positive control

    • Daudi, Jurkat, HepG2 and human brain cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


     This product was previously labelled as HIP2


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab68162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IP 1/20.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
    • Tissue specificity

      Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
      Contains 1 UBA domain.
    • Post-translational

      Sumoylation at Lys-14 impairs catalytic activity.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • E2 25K antibody
      • E2(25K) antibody
      • HIP-2 antibody
      • Huntingtin interacting protein 2 antibody
      • Huntingtin-interacting protein 2 antibody
      • HYPG antibody
      • LIG antibody
      • UBC1 antibody
      • UBE2K antibody
      • UBE2K_HUMAN antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin conjugating enzyme E2 25 kDa antibody
      • ubiquitin conjugating enzyme E2K antibody
      • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
      • Ubiquitin protein ligase antibody
      • Ubiquitin-conjugating enzyme E2 K antibody
      • Ubiquitin-conjugating enzyme E2(25K) antibody
      • Ubiquitin-conjugating enzyme E2-25 kDa antibody
      • Ubiquitin-conjugating enzyme E2-25K antibody
      • Ubiquitin-protein ligase antibody
      see all


    • All lanes : Anti-HIP2/LIG antibody [EP1144Y] (ab68162) at 1/500 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : human brain cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 22 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?


    ab68162 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab68162.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up