Overview

  • Product name

  • Description

    Rabbit polyclonal to HIP55
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 275-325 of Human HIP55 (NP_001014436.1).

  • Positive control

    • HeLa whole cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
    • Sequence similarities

      Belongs to the ABP1 family.
      Contains 1 ADF-H domain.
      Contains 1 SH3 domain.
    • Domain

      The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
    • Post-translational
      modifications

      Degraded by caspases during apoptosis.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
    • Information by UniProt
    • Database links

    • Alternative names

      • ABP1 antibody
      • Actin-binding protein 1 antibody
      • Cervical mucin associated protein antibody
      • Cervical mucin-associated protein antibody
      • Cervical SH3P7 antibody
      • CMAP antibody
      • Dbnl antibody
      • DBNL_HUMAN antibody
      • Drebrin F antibody
      • drebrin like antibody
      • Drebrin like protein antibody
      • Drebrin-F antibody
      • Drebrin-like protein antibody
      • HIP 55 antibody
      • HIP-55 antibody
      • HPK1 interacting protein of 55 kDa antibody
      • HPK1-interacting protein of 55 kDa antibody
      • SH3 domain containing protein 7 antibody
      • SH3 domain-containing protein 7 antibody
      • SH3P7 antibody
      • src homology 3 domain containing protein HIP 55 antibody
      see all

    Images

    • Detection of HIP55 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118794 at 6 µg/mg lysate for IP. For WB detection an anti-HIP55 antibody which recognizes an upstream epitope was used at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 1 second.

    References

    ab118794 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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