Key features and details
- Rabbit polyclonal to HIP55
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HIP55 antibody
See all HIP55 primary antibodies
DescriptionRabbit polyclonal to HIP55
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region within amino acids 275-325 of Human HIP55 (NP_001014436.1).
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab118794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
Sequence similaritiesBelongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
modificationsDegraded by caspases during apoptosis.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
- Information by UniProt
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
Detection of HIP55 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118794 at 6 µg/mg lysate for IP. For WB detection an anti-HIP55 antibody which recognizes an upstream epitope was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab118794 has not yet been referenced specifically in any publications.