Overview

  • Product name

  • Description

    Rabbit polyclonal to HIP55
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human HIP55 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UJU6

  • Positive control

    • IHC-P: Human breast carcinoma tissue. WB: Raji whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.

Target

  • Function

    Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
  • Sequence similarities

    Belongs to the ABP1 family.
    Contains 1 ADF-H domain.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
  • Post-translational
    modifications

    Degraded by caspases during apoptosis.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABP1 antibody
    • Actin-binding protein 1 antibody
    • Cervical mucin associated protein antibody
    • Cervical mucin-associated protein antibody
    • Cervical SH3P7 antibody
    • CMAP antibody
    • Dbnl antibody
    • DBNL_HUMAN antibody
    • Drebrin F antibody
    • drebrin like antibody
    • Drebrin like protein antibody
    • Drebrin-F antibody
    • Drebrin-like protein antibody
    • HIP 55 antibody
    • HIP-55 antibody
    • HPK1 interacting protein of 55 kDa antibody
    • HPK1-interacting protein of 55 kDa antibody
    • SH3 domain containing protein 7 antibody
    • SH3 domain-containing protein 7 antibody
    • SH3P7 antibody
    • src homology 3 domain containing protein HIP 55 antibody
    see all

Images

  • Anti-HIP55 antibody (ab229562) at 1/1000 dilution + Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 48 kDa



    10% SDS-PAGE

  • Paraffin-embedded human breast carcinoma tissue stained for HIP55 with ab229562 at a 1/200 dilution in immunohistochemical analysis.

References

ab229562 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229562.
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