Overview

  • Product name

  • Description

    Goat polyclonal to HIP55
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide:

    AANLSRNGPALQE

    , corresponding to N terminal amino acids 2-14 of Human HIP55.

  • Positive control

    • Jurkat and 3T3 lysates.
  • General notes

    GenBank Accession Number – NP_054782.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22450813
ELISA 1/16000.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa).

Target

  • Function

    Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
  • Sequence similarities

    Belongs to the ABP1 family.
    Contains 1 ADF-H domain.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
  • Post-translational
    modifications

    Degraded by caspases during apoptosis.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABP1 antibody
    • Actin-binding protein 1 antibody
    • Cervical mucin associated protein antibody
    • Cervical mucin-associated protein antibody
    • Cervical SH3P7 antibody
    • CMAP antibody
    • Dbnl antibody
    • DBNL_HUMAN antibody
    • Drebrin F antibody
    • drebrin like antibody
    • Drebrin like protein antibody
    • Drebrin-F antibody
    • Drebrin-like protein antibody
    • HIP 55 antibody
    • HIP-55 antibody
    • HPK1 interacting protein of 55 kDa antibody
    • HPK1-interacting protein of 55 kDa antibody
    • SH3 domain containing protein 7 antibody
    • SH3 domain-containing protein 7 antibody
    • SH3P7 antibody
    • src homology 3 domain containing protein HIP 55 antibody
    see all

Images

  • Anti-HIP55 antibody (ab2836) at 1 µg/ml + 3T3 cell lysate at 35 µg

    Predicted band size: 49 kDa

  • ab2836 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

References

This product has been referenced in:

  • Okur E & Yerlikaya A A novel and effective inhibitor combination involving bortezomib and OTSSP167 for breast cancer cells in light of label-free proteomic analysis. Cell Biol Toxicol N/A:N/A (2018). Read more (PubMed: 29948483) »
  • Lenos KJ  et al. Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer. Nat Cell Biol 20:1193-1202 (2018). Read more (PubMed: 30177776) »
See all 5 Publications for this product

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