Key features and details
- Mouse monoclonal [M334] to HIP55
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HIP55 antibody [M334]
See all HIP55 primary antibodies
DescriptionMouse monoclonal [M334] to HIP55
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to the C-terminal region of Human HIP55
- Jurkat, HeLa or A431 cell lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab86708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa.|
FunctionActin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
Sequence similaritiesBelongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
modificationsDegraded by caspases during apoptosis.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
- Information by UniProt
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
All lanes : Anti-HIP55 antibody [M334] (ab86708) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : A431 cell lysate
Observed band size: 55 kDa why is the actual band size different from the predicted?
ab86708 has been referenced in 1 publication.
- Fish L et al. Muscleblind-like 1 suppresses breast cancer metastatic colonization and stabilizes metastasis suppressor transcripts. Genes Dev 30:386-98 (2016). PubMed: 26883358