Overview

  • Product name

    Anti-HIPK1 antibody - C-terminal
    See all HIPK1 primary antibodies
  • Description

    Rabbit polyclonal to HIPK1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1147-1210 of Human HIPK1 (UniProt ID: Q86Z02).

  • Positive control

    • H1299, HeLa, NIH3T3 and JC whole cell lysates, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab152109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 131 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.
  • Tissue specificity

    Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated and activated by JNK1.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hipk 1 antibody
    • Hipk1 antibody
    • HIPK1_HUMAN antibody
    • Homeodomain interacting protein kinase 1 antibody
    • Homeodomain interacting protein kinase 1 like protein antibody
    • Homeodomain-interacting protein kinase 1 antibody
    • Myak antibody
    • Nbak 2 antibody
    • Nbak2 antibody
    • Nuclear body associated kinase 2 antibody
    • Nuclear body associated kinase 2b antibody
    see all

Images

  • All lanes : Anti-HIPK1 antibody - C-terminal (ab152109) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 131 kDa



    5% SDS PAGE
  • All lanes : Anti-HIPK1 antibody - C-terminal (ab152109) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 131 kDa



    5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling HIPK1 at a 1/200 dilution. Lower image is costained with Hoerchst 33342.

References

This product has been referenced in:

  • Liu B  et al. miR-200c/141 Regulates Breast Cancer Stem Cell Heterogeneity via Targeting HIPK1/ß-Catenin Axis. Theranostics 8:5801-5813 (2018). Read more (PubMed: 30613263) »
See 1 Publication for this product

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