Overview

  • Product name
  • Description
    Rabbit polyclonal to HIPK2
  • Host species
    Rabbit
  • Specificity
    ab28507 recognises HIPK2. It may recognize isoform 1, 2 and 3 of HIPK2 as the immunogen sequence is present in all three isoforms. Please refer to the literature for more information on the three isforms. It does not recognize HIPK1, HIPK3, or HIPK4.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide derived from within residues 37 - 86 of human HIPK2.

    Read Abcam's proprietary immunogen policy .

  • Positive control
    • HepG2 Cell Lysate, human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab28507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/62500.
WB Use a concentration of 0.06 µg/ml. Predicted molecular weight: 110 kDa.Can be blocked with Human HIPK2 peptide (ab111675). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23565059

Target

  • Function
    Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
  • Tissue specificity
    Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosines (By similarity). Autophosphorylated.
    Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
    Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.
  • Cellular localization
    Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • hHIPk 2 antibody
    • hHIPk2 antibody
    • HIPK 2 antibody
    • Hipk2 antibody
    • HIPK2_HUMAN antibody
    • Homeodomain interacting protein kinase 2 antibody
    • Homeodomain-interacting protein kinase 2 antibody
    • Nbak1 antibody
    • Nuclear body-associated kinase 1 antibody
    • PRO0593 antibody
    • Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody
    • Stank antibody
    see all

Images

  • All lanes : Anti-HIPK2 antibody (ab28507) at 1/10000 dilution

    Lane 1 : Control - HeLa Whole Cell extract
    Lane 2 : HIPK2 plasmid overexpression

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : AlexaFluor 680 goat anti-rabbit at 1/10000 dilution

    Predicted band size: 110 kDa



    Samples were incubated in primary antibody for 14 hours at 4°C.

     

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling HIPK2 with ab28507 at 1/100. A Cy3-conjugated donkey anti-rabbit (1/200) was used as the secondary antibody. Positive staining seen the nuclei of pinealocytes and intersticial cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HIPK2. Right - Merge.
  • ab28507, at 4.0-8.0ug/ml, staining human HIPK2 in (Human heart, cells with positive label are Myocardial cells) by Immunohistochemistry, Paraffin embedded tissue.
  • Anti-HIPK2 antibody (ab28507) at 0.06 µg/ml + HepG2 Cell Lysate

    Secondary
    Rabbit Anti HIPK2 at 1/50000 dilution

    Predicted band size: 110 kDa

  • ICC/IF image of ab28507 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28507, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Shang Y  et al. HIPK2-Mediated Transcriptional Control of NMDA Receptor Subunit Expression Regulates Neuronal Survival and Cell Death. J Neurosci 38:4006-4019 (2018). Read more (PubMed: 29581378) »
  • Oh HJ  et al. Inhibition of the processing of miR-25 by HIPK2-Phosphorylated-MeCP2 induces NOX4 in early diabetic nephropathy. Sci Rep 6:38789 (2016). Read more (PubMed: 27941951) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-3 of 3 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (heart rat)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
heart rat
Blocking step
H2O2 as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 12 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 21 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 22 2010

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