Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3819] to HIPK2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-HIPK2 antibody [EPR3819]
See all HIPK2 primary antibodies
DescriptionRabbit monoclonal [EPR3819] to HIPK2
SpecificityThe immunogen used for this product shares 69% homology and 6 consecutive amino acids with HIPK1. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human HIPK2 aa 150-250. The exact sequence is proprietary.
- HT-1080 and HepG2 whole cell lysate (ab7900)
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108543 in the following tested applications.
|WB||1/2000 - 1/10000. Detects a band of approximately 100, 130 kDa (predicted molecular weight: 131 kDa).|
|Flow Cyt||1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProtein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
Tissue specificityHighly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated on tyrosines (By similarity). Autophosphorylated.
Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.
Cellular localizationNucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
- Information by UniProt
- hHIPk 2 antibody
- hHIPk2 antibody
- HIPK 2 antibody
Overlay histogram showing HEK293 cells stained with ab108543 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108543, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
All lanes : Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilution
Lane 1 : HT-1080 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 131 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible isoform)
ab108543 has been referenced in 3 publications.
- Fisher JN et al. MicroRNA networks regulated by all-trans retinoic acid and Lapatinib control the growth, survival and motility of breast cancer cells. Oncotarget 6:13176-200 (2015). WB . PubMed: 25961594
- Cozza G et al. Synthesis and properties of a selective inhibitor of homeodomain-interacting protein kinase 2 (HIPK2). PLoS One 9:e89176 (2014). IP ; Human . PubMed: 24586573
- Dacheux E et al. BRCA1-Dependent Translational Regulation in Breast Cancer Cells. PLoS One 8:e67313 (2013). WB ; Human . PubMed: 23805307