Recombinant
RabMAb

Recombinant Anti-HIPK2 antibody [EPR3819] - BSA and Azide free (ab221980)

Overview

  • Product name

    Anti-HIPK2 antibody [EPR3819] - BSA and Azide free
    See all HIPK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3819] to HIPK2 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 69% homology and 6 consecutive amino acids with HIPK1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: IP, Flow Cyt, ICC, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HIPK2 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HT-1080 and HepG2 cell lysates
  • General notes

    ab221980 is the carrier-free version of ab108543 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab221980 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 100, 130 kDa (predicted molecular weight: 131 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
    • Tissue specificity

      Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosines (By similarity). Autophosphorylated.
      Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
      Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.
    • Cellular localization

      Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
    • Information by UniProt
    • Database links

    • Alternative names

      • hHIPk 2 antibody
      • hHIPk2 antibody
      • HIPK 2 antibody
      • Hipk2 antibody
      • HIPK2_HUMAN antibody
      • Homeodomain interacting protein kinase 2 antibody
      • Homeodomain-interacting protein kinase 2 antibody
      • Nbak1 antibody
      • Nuclear body-associated kinase 1 antibody
      • PRO0593 antibody
      • Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody
      • Stank antibody
      see all

    References

    ab221980 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab221980.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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