Anti-FIST antibody (ab72538)
Key features and details
- Rabbit polyclonal to FIST
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FIST antibody -
Description
Rabbit polyclonal to FIST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human FIST. NM_005734
Database link: Q9H422-1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. -
Tissue specificity
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated, but autophosphorylation is not required for catalytic activity.
May be sumoylated. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10114 Human
- Entrez Gene: 15259 Mouse
- Entrez Gene: 83617 Rat
- Omim: 604424 Human
- SwissProt: Q9H422 Human
- SwissProt: Q9ERH7 Mouse
- SwissProt: O88850 Rat
- Unigene: 201918 Human
see all -
Alternative names
- Androgen receptor interacting nuclear protein kinase antibody
- Androgen receptor-interacting nuclear protein kinase antibody
- ANPK antibody
see all
Images
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ab72538 at 10µg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.
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All lanes : Anti-FIST antibody (ab72538) at 1 µg/ml
Lane 1 : Mouse testis lysate
Lane 2 : Mouse testis lysate with immunising peptide
Lane 3 : Rat testis lysate
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab72538 has been referenced in 2 publications.
- Zhang H et al. ROR1 promotes the proliferation of endometrial cancer cells. Int J Clin Exp Pathol 10:10603-10610 (2017). PubMed: 31966402
- Huh JH et al. Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer. Br J Cancer 109:452-61 (2013). PubMed: 23807165