Product nameAnti-HIPK4 antibody
DescriptionRabbit polyclonal to HIPK4
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from an internal internal of human HIPK4.
- A549 clls; HUVEC cells; testis tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionProtein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- HIPK 4 antibody
- HIPK4 antibody
- HIPK4_HUMAN antibody
All lanes : Anti-HIPK4 antibody (ab69565) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Immunohistochemistry analysis of paraffin-embedded human testis tissue using 1:50 ab69565. For the right hand image the antibody was pre-incubated with the immunizing peptide.
Immunofluorescence analysis of A549 cells, using 1:500 ab69565. For the right hand image, the antibody was pre-incubated with the immunizing peptide.
ab69565 has not yet been referenced specifically in any publications.