Overview

  • Product name

  • Description

    Goat polyclonal to HIPPI/IFT57
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Rabbit, Horse, Chicken, Hamster, Pig, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to Human HIPPI/IFT57 aa 103-117 (internal sequence) (Cysteine residue). (AAK28373.1)
    Sequence:

    EQPQEYDDPNATISN

  • Positive control

    • Human testis tissue; Mouse brain lysates.
  • General notes

     This product was previously labelled as HIPPI

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 49 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/8000.

Target

  • Function

    Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
  • Tissue specificity

    Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
  • Sequence similarities

    Belongs to the IFT57 family.
  • Domain

    The pseudo DED region (pDED) meadiates the interaction with HIP1.
  • Cellular localization

    Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDNA FLJ10147 fis clone HEMBA1003369 antibody
    • Dermal papilla derived protein 8 antibody
    • Dermal papilla-derived protein 8 antibody
    • DERP 8 antibody
    • DERP8 antibody
    • Esrrbl 1 antibody
    • Esrrbl1 antibody
    • ESRRBL1 protein antibody
    • Estrogen related receptor beta like 1 antibody
    • Estrogen related receptor beta like protein 1 antibody
    • Estrogen-related receptor beta-like protein 1 antibody
    • FLJ10147 antibody
    • Hip1 Interacting protein antibody
    • HIP1 interacting protein HIPPI antibody
    • Hip1 protein interactor antibody
    • HIP1-interacting protein antibody
    • HIPPI antibody
    • Huntingtin interacting protein 1 interacting protein antibody
    • Huntingtin interacting protein 1 protein interactor antibody
    • IFT 57 antibody
    • ift57 antibody
    • IFT57_HUMAN antibody
    • Intraflagellar transport 57 homolog (Chlamydomonas) antibody
    • Intraflagellar transport 57 homolog antibody
    • Intraflagellar transport protein 57 homolog antibody
    • MHS4R2 antibody
    • Vestrogen related receptor beta like 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labelling HIPPI/IFT57 with ab140842 at 5 µg/ml.

  • Anti-HIPPI/IFT57 antibody (ab140842) at 1 µg/ml + Mouse brain lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 49 kDa

References

ab140842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab140842.
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