Anti-HIPPI/IFT57 antibody (ab154601)
Key features and details
- Rabbit polyclonal to HIPPI/IFT57
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIPPI/IFT57 antibody -
Description
Rabbit polyclonal to HIPPI/IFT57 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human HIPPI/IFT57 aa 227-429.
Database link: Q9NWB7 -
Positive control
- 293T, A431, H1299, HeLa, HepG2 and Raji whole cell lysates. HeLa cells.
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General notes
Keep as concentrated solution.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear. -
Tissue specificity
Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level). -
Sequence similarities
Belongs to the IFT57 family. -
Domain
The pseudo DED region (pDED) meadiates the interaction with HIP1. -
Cellular localization
Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia. - Information by UniProt
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Database links
- Entrez Gene: 531436 Cow
- Entrez Gene: 55081 Human
- Entrez Gene: 73916 Mouse
- Entrez Gene: 303968 Rat
- Omim: 606621 Human
- SwissProt: Q5EA95 Cow
- SwissProt: Q9NWB7 Human
- SwissProt: Q8BXG3 Mouse
see all -
Alternative names
- CDNA FLJ10147 fis clone HEMBA1003369 antibody
- Dermal papilla derived protein 8 antibody
- Dermal papilla-derived protein 8 antibody
see all
Images
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Anti-HIPPI/IFT57 antibody (ab154601) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling HIPPI/IFT57 with ab154601 at 1/500 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling HIPPI/IFT57 with ab154601 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154601 has not yet been referenced specifically in any publications.