Overview

  • Product name

  • Description

    Rabbit polyclonal to HIPPI/IFT57
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human HIPPI/IFT57 aa 227-429.
    Database link: Q9NWB7

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2 and Raji whole cell lysates. HeLa cells.
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as HIPPI

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
  • Tissue specificity

    Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
  • Sequence similarities

    Belongs to the IFT57 family.
  • Domain

    The pseudo DED region (pDED) meadiates the interaction with HIP1.
  • Cellular localization

    Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDNA FLJ10147 fis clone HEMBA1003369 antibody
    • Dermal papilla derived protein 8 antibody
    • Dermal papilla-derived protein 8 antibody
    • DERP 8 antibody
    • DERP8 antibody
    • Esrrbl 1 antibody
    • Esrrbl1 antibody
    • ESRRBL1 protein antibody
    • Estrogen related receptor beta like 1 antibody
    • Estrogen related receptor beta like protein 1 antibody
    • Estrogen-related receptor beta-like protein 1 antibody
    • FLJ10147 antibody
    • Hip1 Interacting protein antibody
    • HIP1 interacting protein HIPPI antibody
    • Hip1 protein interactor antibody
    • HIP1-interacting protein antibody
    • HIPPI antibody
    • Huntingtin interacting protein 1 interacting protein antibody
    • Huntingtin interacting protein 1 protein interactor antibody
    • IFT 57 antibody
    • ift57 antibody
    • IFT57_HUMAN antibody
    • Intraflagellar transport 57 homolog (Chlamydomonas) antibody
    • Intraflagellar transport 57 homolog antibody
    • Intraflagellar transport protein 57 homolog antibody
    • MHS4R2 antibody
    • Vestrogen related receptor beta like 1 antibody
    see all

Images

  • Anti-HIPPI/IFT57 antibody (ab154601) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling HIPPI/IFT57 with ab154601 at 1/500 dilution.

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling HIPPI/IFT57 with ab154601 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154601 has not yet been referenced specifically in any publications.

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